Serveur d'exploration sur le phanerochaete

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Characterization of the mnp2 gene encoding manganese peroxidase isozyme 2 from the basidiomycete Phanerochaete chrysosporium.

Identifieur interne : 000D62 ( Main/Exploration ); précédent : 000D61; suivant : 000D63

Characterization of the mnp2 gene encoding manganese peroxidase isozyme 2 from the basidiomycete Phanerochaete chrysosporium.

Auteurs : M B Mayfield ; B J Godfrey ; M H Gold

Source :

RBID : pubmed:8194756

Descripteurs français

English descriptors

Abstract

The nucleotide (nt) sequence of a gene (mnp2) encoding manganese peroxidase isozyme 2 (MnP-2) from Phanerochaete chrysosporium was determined. The sequence of 3297 bp includes 1287 bp of 5'-flanking sequence and 490 bp 3' to the stop codon. Comparison of cDNA and genomic sequences indicates seven introns varying in size from 50-55 bp. The 5' upstream region of the mnp2 gene contains a TATAA element, three inverted CCAAT elements (ATTGG), six putative heat-shock elements (HSE) and three putative metal response elements (MRE) (TGCRCNC), all located within 1100-bp upstream from the start codon. The positions of the putative HSE and MRE in the promoter region of mnp2 are compared with the corresponding sequences in the mnp1 gene promoter. A Northern blot probed with a fragment specific for the mnp2 gene suggests that the transcription of mnp2 is regulated by Mn ions.

DOI: 10.1016/0378-1119(94)90266-6
PubMed: 8194756


Affiliations:


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Le document en format XML

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<title xml:lang="en">Characterization of the mnp2 gene encoding manganese peroxidase isozyme 2 from the basidiomycete Phanerochaete chrysosporium.</title>
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<name sortKey="Mayfield, M B" sort="Mayfield, M B" uniqKey="Mayfield M" first="M B" last="Mayfield">M B Mayfield</name>
<affiliation>
<nlm:affiliation>Department of Chemistry, Biochemistry, and Molecular Biology, Oregon Graduate Institute of Science & Technology, Portland 97291-1000.</nlm:affiliation>
<wicri:noCountry code="subField">Portland 97291-1000</wicri:noCountry>
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<name sortKey="Godfrey, B J" sort="Godfrey, B J" uniqKey="Godfrey B" first="B J" last="Godfrey">B J Godfrey</name>
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<author>
<name sortKey="Gold, M H" sort="Gold, M H" uniqKey="Gold M" first="M H" last="Gold">M H Gold</name>
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<term>Amino Acid Sequence (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Basidiomycota (enzymology)</term>
<term>Basidiomycota (genetics)</term>
<term>DNA, Complementary (analysis)</term>
<term>DNA, Fungal (analysis)</term>
<term>Gene Expression Regulation, Fungal (genetics)</term>
<term>Genes, Fungal (genetics)</term>
<term>Isoenzymes (chemistry)</term>
<term>Isoenzymes (genetics)</term>
<term>Manganese Compounds (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Peroxidases (chemistry)</term>
<term>Peroxidases (genetics)</term>
<term>Protein Sorting Signals (genetics)</term>
<term>RNA, Messenger (analysis)</term>
<term>Regulatory Sequences, Nucleic Acid (genetics)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Sulfates (MeSH)</term>
<term>Transcription, Genetic (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>ADN complémentaire (analyse)</term>
<term>ADN fongique (analyse)</term>
<term>ARN messager (analyse)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Basidiomycota (enzymologie)</term>
<term>Basidiomycota (génétique)</term>
<term>Composés du manganèse (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Gènes fongiques (génétique)</term>
<term>Isoenzymes (composition chimique)</term>
<term>Isoenzymes (génétique)</term>
<term>Peroxidases (composition chimique)</term>
<term>Peroxidases (génétique)</term>
<term>Régulation de l'expression des gènes fongiques (génétique)</term>
<term>Signaux de triage des protéines (génétique)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Sulfates (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Séquences d'acides nucléiques régulatrices (génétique)</term>
<term>Transcription génétique (MeSH)</term>
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<term>DNA, Complementary</term>
<term>DNA, Fungal</term>
<term>RNA, Messenger</term>
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<term>Isoenzymes</term>
<term>Peroxidases</term>
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<term>ADN fongique</term>
<term>ARN messager</term>
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<term>Isoenzymes</term>
<term>Peroxidases</term>
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<term>Basidiomycota</term>
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<term>Basidiomycota</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Basidiomycota</term>
<term>Gene Expression Regulation, Fungal</term>
<term>Genes, Fungal</term>
<term>Isoenzymes</term>
<term>Peroxidases</term>
<term>Protein Sorting Signals</term>
<term>Regulatory Sequences, Nucleic Acid</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr">
<term>Basidiomycota</term>
<term>Gènes fongiques</term>
<term>Isoenzymes</term>
<term>Peroxidases</term>
<term>Régulation de l'expression des gènes fongiques</term>
<term>Signaux de triage des protéines</term>
<term>Séquences d'acides nucléiques régulatrices</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Manganese Compounds</term>
<term>Molecular Sequence Data</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
<term>Sulfates</term>
<term>Transcription, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr">
<term>Analyse de séquence d'ADN</term>
<term>Composés du manganèse</term>
<term>Données de séquences moléculaires</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Sulfates</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Transcription génétique</term>
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<front>
<div type="abstract" xml:lang="en">The nucleotide (nt) sequence of a gene (mnp2) encoding manganese peroxidase isozyme 2 (MnP-2) from Phanerochaete chrysosporium was determined. The sequence of 3297 bp includes 1287 bp of 5'-flanking sequence and 490 bp 3' to the stop codon. Comparison of cDNA and genomic sequences indicates seven introns varying in size from 50-55 bp. The 5' upstream region of the mnp2 gene contains a TATAA element, three inverted CCAAT elements (ATTGG), six putative heat-shock elements (HSE) and three putative metal response elements (MRE) (TGCRCNC), all located within 1100-bp upstream from the start codon. The positions of the putative HSE and MRE in the promoter region of mnp2 are compared with the corresponding sequences in the mnp1 gene promoter. A Northern blot probed with a fragment specific for the mnp2 gene suggests that the transcription of mnp2 is regulated by Mn ions.</div>
</front>
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<Month>06</Month>
<Day>29</Day>
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<DateRevised>
<Year>2019</Year>
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<Day>07</Day>
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<Issue>2</Issue>
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<Title>Gene</Title>
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<ArticleTitle>Characterization of the mnp2 gene encoding manganese peroxidase isozyme 2 from the basidiomycete Phanerochaete chrysosporium.</ArticleTitle>
<Pagination>
<MedlinePgn>231-5</MedlinePgn>
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<Abstract>
<AbstractText>The nucleotide (nt) sequence of a gene (mnp2) encoding manganese peroxidase isozyme 2 (MnP-2) from Phanerochaete chrysosporium was determined. The sequence of 3297 bp includes 1287 bp of 5'-flanking sequence and 490 bp 3' to the stop codon. Comparison of cDNA and genomic sequences indicates seven introns varying in size from 50-55 bp. The 5' upstream region of the mnp2 gene contains a TATAA element, three inverted CCAAT elements (ATTGG), six putative heat-shock elements (HSE) and three putative metal response elements (MRE) (TGCRCNC), all located within 1100-bp upstream from the start codon. The positions of the putative HSE and MRE in the promoter region of mnp2 are compared with the corresponding sequences in the mnp1 gene promoter. A Northern blot probed with a fragment specific for the mnp2 gene suggests that the transcription of mnp2 is regulated by Mn ions.</AbstractText>
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<Chemical>
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<NameOfSubstance UI="D017895">Manganese Compounds</NameOfSubstance>
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<DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
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<DescriptorName UI="D017895" MajorTopicYN="N">Manganese Compounds</DescriptorName>
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<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
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<DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName>
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